The human perception that has multiple origins is influenced by external and internal factors. Some of these effects cause distortion in knowledge and others cause acceleration or create a new kind of knowledge and insight. From the point of view of verses and hadiths and from the viewpoint of moral scholars and sages, virtues and vices are considered as some of the factors affecting human knowledge. This influence can sometimes be proved as a desire to acquire knowledge or to accelerate understanding or create some enlightened perceptions due to virtuousness and sometimes in distorting or depriving of some types of perceptions due to viciousness. Of course, the degree of these effects and their intensity and weakness are different owing to different perceptions. The problem we are trying to solve in this article is how to analyze and study this impact in a rational way. According to the authors of this article, through an analytical method, it is possible to present a rational explanation of the effects of virtues and vices on human knowledge by “analyzing the basic humanities of philosophical ethics”, “the necessity of the compatibility between cause and effect”, “the existence of a relationship between emotions and knowledge” and finally “the agency of virtues and vices for the enlightenment and darkness of the self”.